Protection of DNA from oxidative damage by purified proteins in the MFO system.

<p>Lane 1, pUC19 plasmid DNA only; lane 2, pUC19 plasmid DNA + FeCl₃; lane 3, pUC19 plasmid DNA + FeCl₃ + DTT; lane 4–10, pUC19 plasmid DNA + FeCl₃ + DTT + purified proteins (5, 10, 20, 40, 60, 80, and 100 μg/mL, respectively). SF, supercoiled form; NF, nicked form. SF% is SF/(SF + NF) for each lane.The expression level was determined by the signal intensities of the bands.