Agarose gel electropherogram.

<p>(A) Polyplexes at various N/P ratios without serum: (a) CS/pDNA, (b) CS-<i>g</i>-PEI-0.9/pDNA, (c) CS-<i>g</i>-PEI-1.8/pDNA, (d) CS-<i>g</i>-PEI-4.5/pDNA, and (e) CS-<i>g</i>-PEI-9.0/pDNA. Lane 0: DNA markers, lanes1-4: N/P ratios of 1, 2, 4 and 6, respectively. (B) Polyplexes at the N/P ratio of 6 in 10%, 25%, 50% serum, respectively. Lane 0: DNA markers, lane 0’: pDNA, lane 1: CS/pDNA, lane 2: CS-<i>g</i>-PEI-0.9/pDNA, lane 3: CS-<i>g</i>-PEI-1.8/pDNA, lane 4: CS-<i>g</i>-PEI-4.5/pDNA, lane 5: CS-<i>g</i>-PEI-9.0/pDNA.