The effect of NUCB2^1–83 on osteoclast differentiation quantified by measuring TRAP activity.

<p>RAW 264.7 cells were cultured in the presence of RANKL (50 ng/mL) for 5 days with or without NUCB2^1–83. The cells were fixed and incubated in 10 mM citrate buffer (pH 4.6) containing 10 mM sodium tratrate and 5 mM p-nitrophenylphosphate for 1 h followed by transferring into new well plates containing an equal volume of 0.1 N NaOH. TRAP activity was measured at λ = 405 nm and expressed as percent of that of untreated control. Data represented the mean±SEM(*P<0.05). Number of samples used is showed within the parentheses.