Illustrative Inhibition studies of anti-PLA₂R antibodies using synthetic peptides with an anti-PLA₂R positive serum sample.

<p><i>Panel A:</i> Different concentrations of a mixture of PLA₂R derived peptides were used to inhibit the reactivity to the PLA₂R whole molecule in an addressable laser bead assay. The reactivity showed a significant, dose dependent inhibition. The inhibition with a control peptide (GE-1) was significantly lower. All values are expressed as residual reactivity after inhibition (in %) compared to the sample without inhibitor or control. <i>Panel B:</i> The peptide mixture together with seven individual PLA₂R peptides and a control peptide were used at a concentration of 126 µg/mL. Besides the peptide mixture, peptide 3 and peptide 4 showed inhibition of anti-PLA₂R antibodies. All values are expressed as residual reactivity after inhibition (in %) compared to the sample without inhibitor or control. <i>Panel C:</i> The peptide mixture together with seven individual PLA₂R peptides and a control peptide were used at a concentration of 126 µg/mL. Besides the peptide mixture, peptide 3 and peptide 4 showed inhibition of anti-PLA₂R antibodies. All values are expressed as ALBIA median fluorescence intensities (MFI) or titer by indirect immunofluorescence on cell-based assay (IIF-CBA).