Binding of purified anti-PsA peptide antibodies to autoantigens.

<p><b>A.</b> Binding of affinity purified anti-PsA peptide antibodies to solid phase fibrillin 3, desmocollin 3, keratin 78 and N-RAP peptide in DELFIA assay. Fibrillin, desmocollin and keratin are commercially available recombinant molecules. Binding of antibodies to N-RAP was assessed using solid phase N-RAP peptide. The pink bars represent the means of the 5 individual affinity purified anti-PsA peptide antibodies (10 microgram/ml) purified from 5 different PsA patients, the blue bars represent the means of the 5 control antibody populations affinity purified directed against irrelevant peptide from the sera of the same 5 PsA patients. Error bars represent the standard deviations obtained, each test was perfomed in triplicate and repeated three independent times. Y axis absorbance values. <b>B.</b> Binding of affinity purified anti-PsA peptide antibodies to solid phase IL17B (see above).