Epitope Mapping of PLA₂R Peptides with anti-human IgG₄.

<p>Grey scale heat map representation of results from SPOT used to detect PLA₂R epitopes. Peptide membranes were probed with 10 randomly selected IMN samples that had previously been tested by IIF-CBA for anti-PLA₂R antibodies (7 positive (IMN+) and 3 negative (IMN-)), as well as 5 normal healthy controls (NHC). The positive control rabbit antibody to PLA₂R reacted with the expected peptide used as the immunogen. Consensus epitopes and their respective PLA₂R domains derived from this analysis are illustrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061669#pone-0061669-g003" target="_blank">Figure 3</a> and summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061669#pone-0061669-t001" target="_blank">Table 1</a>.