SDS-PAGE profile and immunoblot of 31 kDa kidney bean protein.

<p>Lane 1 : untreated, Lane 2−5 : boiled at 100°C for 15, 30, 45 and 60 min, Lane M: molecular weight markers. Protein fractions were stained with Coomassie brilliant blue (A) and immunoblotted (B). SGF digestion of 31 kDa protein Lane M:molecular weight markers, Lane 1: 31 kDa protein (undigested), lanes 2−8: 31 kDa protein incubated in SGF for 0.5, 1, 5, 10, 15, 30 and 60 min, lane 9: pepsin. The digested protein was electrophoresed on SDS-PAGE and visualized by CBB staining (C).