Identification and construction of pcDNA3.1-GLUT1 and pcDNA3.1-GLUT12.

<p>(A) Identification of pcDNA3.1-GLUT1 by digestion with <i>Nhe</i> I and <i>Hind</i> III. M1: DL5000 DNA Marker, pCG1: inserted into pcDNA3.1, pCG1 <i>Nhe</i> I + <i>Hind</i> III: digested with <i>Nhe</i> I and <i>Hind</i> III. (B) PCR analysis using primers GLUT1-F/GLUT1-R. M2: Trans2KTM Plus DNA Marker, GLUT1: 1481bp PCR production of goat GLUT1. (C) pcDNA3.1-GLUT1 vector construction diagram. (D) PCR analysis using primers GLUT12-F2/GLUT12-R2. M1: DNA Marker, GLUT12: 1866bp PCR production of goat GLUT12. (E) Identification of pcDNA3.1-GLUT12 by digestion with <i>Xho</i> I. M2: Trans2K plus DNA Marker, pCG12 <i>Xho</i> I: digested with <i>Xho</i> I. (F) pcDNA3.1-GLUT12 vector construction diagram.