Verification of the integration sites of the transgene by PCR.

<p>(A) The arrows represent the PCR primers used for the detection of the flanking sequences. G1 and G3 are genome-specific primers, and T2 and T4 are transgene-specific primers. WT, genome of wild-type cattle; hLF, genome of transgenic cattle. (B) PCR detection of the integration sites in the three transgenic cattle and one wild-type cow. The amplified product for the wild-type sequence was 633 bp, while those for the 5′ and 3′ flanking regions of the transgenic sequence were 511 bp and 422 bp, respectively. M, 100 bp DNA ladder; WT, genome of wide-type cattle, which was used as the negative control.