PCR primers used to generate DNA constructs.

<p>The PCR primers are listed in pairs, with the forward primer (F) listed first and the reverse primer (R) second. All PCR products were cloned into pCDNA3.1-mycSV40, and the restriction enzyme sites used for cloning are underlined.</p>*<p>indicates that these primers have a +1 nucleotide after the restriction site to put the gene in frame with the tag.