Elevation of DSG3 is associated with plakoglobin translocation in cancer tissues.

<p>(<b>A</b>) Cells from two clones of normal keratinocytes (HNK1, HNK2) and two cancer cell lines (OECM1, SAS) were examined. (B) Clinical tissue samples of four grossly normal biopsies and five pieces of cancer tissues from head-neck cancer patients were examined. Cellular or nuclear proteins were extracted and subjected to immunoblot analysis for the expression of DSG3, plakoglobin, c-myc, Cyclin D1 and MMP7, as described in the method section. Expressions of Actin served as internal control for cellular protein, and HDAC for nuclear protein. Cellular protein expression was normalized by Actin and nuclear protein by HDAC after determination of each band density by Image J software.