GSK3β is overexpressed and active in NSCLC tumor tissues.

<p>(<b>A</b>) Typical IHC staining images of human NSCLC tumor and normal lung tissue (magnification × 200). (<b>B</b>) Western blotting assays confirmed that in paired normal/tumor tissues from NSCLC cancer patients, higher expression levels of GSK3β in tumors were found compared with their normal counterparts. The experiments were repeated 3 times, and the data are presented as the mean ± SD. *<i>P</i><0.001. (<b>C</b>) Phosphorylated glycogen synthase (pGS), a substrate of GSK3β, was detected in these NSCLC cell lines, except for H292.