Reduced expression of KCC2 mRNA within the SNr following TAA injection.

<p>Real-time quantitative RT-PCR experiments were performed using SYBR green for analysis of KCC2 (A and B) and NKCC1 (C and D) mRNAs expression in SNr of TAA-injected mice at different time points and sham controls. All data were normalized for levels of GAPDH expression within the same sample. Data are calculated as percentages of the average value of controls (B and D). Note that a dramatic down-regulation of KCC2 mRNA was seen at 1 d and maintained up to 3 d in TAA group (B). However, a significant increase in NKCC1 mRNA was observed at 1 d in both the sham and TAA groups, which disappeared at 2 d and 3 d after treatment (D). * <i>P</i><0.05 vs sham controls.