Changes in <i>Ikgkb</i> (<i>Nemo</i>) mRNA expression in control (Ctrl) and pCX-TRI-2A-transfected cells.

<p>Cells were incubated with 50 ng/ml TNF-α for 16h, alone or in combination with 20 μM hemin and/or 200 μM ATP. Murine <i>Ikgkb</i> mRNA was quantified by real-time PCR. The data (mean±SD of three independent experiments), normalized for <i>Gapdh</i> gene, are expressed as fold change respect to the untreated cells. [*] indicates a significant difference between pCX-TRI-2A-transfected cells and Ctrl cells within the same treatment (t Student, <i>p</i><0.05); [#] indicates a significant difference as compared to untreated cells within the same cell type (ANOVA, <i>p</i><0.05) [a] indicates a significant difference as compared to TNF-α treatment alone within the same cell type (ANOVA, <i>p</i><0.05).