Neuroprotection by 2-BFI against NMDA toxicity.

<p>(A–E) Photomicrographs of nuclei of cortical neurons treated with or without NMDA and other indicated inhibitiors. Arrows show condensed nuclei, indicating dead cells. Scale bar = 100 µm. (F) Treated cortical neurons were subjected to Western blotting to show inhibition of the production of spectrin breakdown product (SBP) induced by NMDA excitotoxicity. GAPDH was used as an internal protein loading control. (G) Dose-dependent neuroprotection against NMDA by 2-BFI was measured using an Alamar blue assay the mean ± SEM was plotted (n = 3). (H) Time-dependent protection by 2-BFI against NMDA toxicity to cortical neurons was measured using an Alamar blue assay. The data represents mean ± SEM of at least three repeats. ** indicates statistical significant using paired t-test (<i>P</i><0.01).