A quantitative method to assess extrasynaptic NMDA receptor function in the protective effect of synaptic activity against neurotoxicity-2

R 10 min. Five hours after NMDA washout the cells were fixed and stained with Hoechst 33258. Arrowheads indicate dead cells. Scale bar is equal to 10 μm. (B) Evaluation of neuronal cultures following treatment with bicuculline and NMDA showed the protective effect of bicuculline pretreatment on NMDA induced cell death. The graph represents the mean and SEM of five independent experiments. *** p < 0.001 independent samples t-test.<p><b>Copyright information:</b></p><p>Taken from "A quantitative method to assess extrasynaptic NMDA receptor function in the protective effect of synaptic activity against neurotoxicity"</p><p>http://www.biomedcentral.com/1471-2202/9/11</p><p>BMC Neuroscience 2008;9():11-11.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267199.</p><p>