FTY720-P suppressed oAβ–induced neurotoxicity.

<p>(A) Western blotting image for characterization of oAβ ˜ Data showed Aβ1-42 oligomerization composed with small (monomer, dimer, 3-mer, 4-mer, 8-mer, and 12-mer) and large oligomers. (B) Fluorescent microscopic images of mouse primary cortical neuron cultures. Treatment with FTY720-P was neuroprotective against oAβ–mediated toxicity. Neurons were stained with anti–MAP-2 (green) antibodies and Aβ was stained with 4G8 antibodies` (red). Scale bar: 50 µm. NT, no treatment; oA 5 µM oAβ1-42treatment. (C) Relative neuronal survival. The number of viable neurons (MAP-2–positive neurons) was quantified relative to results observed with untreated neurons. FTY720-P rescued neurons against oAβ–mediated toxicity. oA 5 µM oAβ1-42treatment. *, <i>P</i><0.001; †, <i>P</i><0.001. Values are means±SEM (n = 3). (D) WST-8 assay. FTY720-P enhanced neuronal survival against oAβ–mediated toxicity. oA 5 µM oAβ1-42treatment. *, <i>P</i><0.001; †, <i>P</i><0.001. Values are means ± SEM (n = 6).