Specificity of the modified primer pair.

<p>Agarose gels showing the amplification products from polymerase chain reaction (PCR) using genomic DNA from isolates of 15 <i>formae speciales</i>, including <i>basilici</i>, <i>chrysanthemi</i>, <i>cubense</i>, <i>cucumerinum</i>, <i>radicis-cucumerinum</i>, <i>gladioli</i>, <i>lactucae</i>, <i>lilii</i>, <i>luffae</i>, <i>lycopersici</i>, <i>melonis</i>, <i>momordicae</i>, <i>niveum</i>, <i>phaseoli</i> and <i>tracheiphilum</i>, and five nonpathogenic strains of <i>Fusarium oxysporum</i> (<i>Fo</i>). (A) 550 to 650 bp DNA products of different <i>formae speciles</i> and nonpathogenic <i>Fo</i> isolates amplified by FIGS11/FIGS12. (B) 500-bp DNA product of five nonpathogenic <i>Fo</i> isolates amplified by new primer NPIGS-R and FIGS11. The numbers on the left are the molecular weights (Kb) of the Gen-100 bp DNA ladder (GeneMark) (lane M).