Sensitivity of the newly designed primer.

<p>Agarose gel showing the sensitivity of polymerase chain reaction (PCR) using the genomic DNA of a nonpathogenic strain of <i>Fusarium oxysporum</i> and the primer pair FIGS11/NPIGS-R: Amplification of a decreasing amount of the nonpathogenic isolate Fo276 DNA ranging from 200 to 10^-3 ng. The numbers on the left correspond to the molecular weight (kb) of the Gen-100 ladder (lane M). Lanes Foc16416 and Fo366, the amplification controls for the pathogenic isolate of <i>F. oxysporum</i> f. sp. <i>cucumerinum</i> (Foc100) and nonpathogenic isolate Fo366 DNA, respectively. Lane C, control reaction with no template DNA.