NLGP normalizes protumor angiogenic and hypoxic TME.

<p>Sarcoma 180 tumor tissue (100 mg) harvested from Swiss albino mice was lysed by freeze-thaw technique in 1 ml PBS supplemented with a cocktail of protease inhibitors. <b>A.</b> Tumor tissue lysates, representing TME from either PBS or NLGP treated mice (n = 6 in each case) were assessed for TGFβ and VEGF by ELISA. Cytokines were quantitated as pg/mg of tissue ± SE. *<i>p<</i>0.001, **<i>p<</i>0.01, in comparison to PBS treated tumor on day 15 and 20. <b>B.</b> Total protein was isolated from PBS and NLGP treated tumors (n = 3 in each case) to assess TGFβ, VEGF, HIF1α, VEGFR1, VEGFR2 and β-actin by Western blot analysis <b>C.1.</b> Total RNA was isolated from tumors of PBS and NLGP treated mice (n = 3 in each case) to analyze genes, like, VEGF, HIF1α, VEGFR1, VEGFR2 and TGFβ at transcriptional level by RT-PCR <b>C.2.</b> Densitometric analysis was performed in each case. Frozen sections of tumors from either PBS or NLGP treated mice were stained <b>D.1.</b> immunohistochemically with monoclonal antibodies, specific for VEGF, VEGFR1, VEGFR2, HIF1α and TGFβ and <b>D.2.</b> with fluorescence tagged anti-CD31 antibody <b>E.1.</b> Total RNA from tumors of PBS and NLGP treated mice was used to determine the status of perforin, granzyme B on day 15 and 20 of tumor inoculation. <b>E.2.</b> Densitometric analysis was performed in each case.