Western blotting of virion and cell lysate samples collected from cells transfected under various conditions.

<p>The amount of envelope plasmid in μg is indicated above the lanes. The double bands from anti-gp120 staining corresponded to gp120 (bottom) and gp160 (top), respectively. β-actin expression was also probed simultaneously to validate the comparison across different transfection conditions. Two independent analyses yielded results that support the same conclusions.