Western blot analysis of co-immunoprecipitated proteins (D2ET and D2E12).

<p>HEK 293T/17 cells were transfected with D2ET or D2E12 and cell lysates were collected on day 2 post transfection. (A) Immunoprecipitation was performed using an anti-actin antibody and western analysis was undertaken with an anti-FLAG (anti-OctA-Probe) antibody. (B) Filter from (A) was stripped and re-probed with an anti-actin antibody to confirm immunoprecipitation of actin. (C) Immunoprecipitation was performed using an anti-FLAG (anti-OctA-Probe) antibody and western analysis was undertaken with an anti-actin antibody. (D) Filter from (C) was stripped and re-probed with an anti-FLAG (anti-OctA-Probe) antibody to confirm immunoprecipitation of FLAG-tagged constructs.