Results of co-immunoprecipitation.

<p>Sf9 cells were transfected with plasmids expressing VP37-FLAG (FLAG-tagged VP37), LvPT-V5 (V5-tagged LvPT) or empty plasmid (vector). At 6 h after heat shock, the cell lysates were harvested. (A) After separation by SDS-PAGE, expression of VP37-FLAG and LvPT-V5 was confirmed by western blot using anti-His antibody as a probe. Arrows indicate the expressed VP37-FLAG and LvPT-V5. (B) The cell lysates were immunoprecipitated with anti-FLAG M2 affinity resins and then the immunoprecipitated complexes were subjected to western blot analysis with anti-His antibody probe.