Binding of UvsX to double-stranded DNA in the absence or presence of nucleotide cofactors.

<p>Reactions were initiated by the addition of UvsX (final concentrations indicated on graph) to dsDNA, oligonucleotide dT₂₅:dA₂₂XA₂ (oligo 2:oligo 1) and nucleotide cofactors final concentrations are indicated below. The amplitude of fluorescence quenching was graphed as a function of UvsX concentrations and fit to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066654#pone.0066654.e001" target="_blank">Equation 1</a> to determine an apparent <i>K_d</i> value. (A) 0.5 µM (nucleotide pairs) dsDNA (B) 2 µM dsDNA and 2.5 mM ATP (C) 2 µM dsDNA and 900 µM ATPγS.