Fluorescence intensity of oligonucleotides hybridized products with 1× PicoGreen after S1 nuclease-mediated cleavage.

<p>The concentration of oligonucleotides and their complementary oligonucleotides is 50 nM and 100 nM respectively. (A) The effects of lengths of target ssDNA on fluorescence intensity. (B) The effects of nucleotide composition of target ssDNA on fluorescence intensity. (C) Linearity of the proposed method (three virus ssDNA). Plots of fluorescence intensity versus different concentrations of target ssDNA hybridized products (linear range, 50 pM–64 nM).