Detection results of Sequence-specificity ssDNA in different surroundings.

<p>(A). Fluorescence intensities of three viruses ssDNA and their hybridized products after S1 nuclease-mediated cleavage using SYBR Gold (1×). The concentration of target ssDNAs are 50 nM, and their complementary ssDNAs are 100 nM. a, H1N1-dsDNA; b, CaM-dsDNA; c, HCV-dsDNA; d, H1N1-ssDNA; e, CaM-ssDNA; f, HCV-ssDNA; g, blank. (B). Fluorescence intensities of three viruses ssDNA and their hybridized products in 4% pork muscle tissue homogenate after S1 nuclease-mediated cleavage using SYBR Gold (1×). The concentration of target ssDNAs are 50 nM, and their complementary ssDNAs are 100 nM. (C). Fluorescence intensities of 50 pM HCV-ssDNA and its hybridized products in 0.5 µg·mL⁻¹ Lambda DNA standard solution after S1 nuclease-mediated cleavage using SYBR Gold (1×). (D). Fluorescence intensities of 50 nM HCV-ssDNA and its hybridized products in 5 µg·mL⁻¹ Lambda DNA standard solution after S1 nuclease-mediated cleavage using SYBR Gold (1×).