Determination of DNA binding stoichiometries.

<p><b>(A-C).</b> Determination of stoichiometry of oligonucleotide binding to WCGp80, using oligomers of different sizes 17, 41, 60. ATPase assays as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134762#pone.0134762.g003" target="_blank">Fig 3</a> were performed with a higher concentration (8 μM) of the enzyme resulting in titration curves. The junction between the lines drawn through the rising and horizontal section is the point where equivalence is reached. <b>(D),</b> The estimated number of subunits bound to the oligonucleotides was plotted against their sizes. The best- fit straight line through the points is shown. <b>(E),</b> Stoichiometric titrations using the 41 mer oligonucleotide. Fluorescence quenching experiments were performed to determine DNA binding by the protein, WCGp80. In this experiment, the protein concentration used was 400 nM. The junction between the straight lines drawn, through the rising and horizontal parts of the graphs indicates the breakpoint.