Co-localization and interaction of MARCH2 with STX6 and CAL.

<p>(<b>A</b>) HEK293 cells grown on coverslips were co-transfected with 3µg GFP-STX6 or 3µg GFP-CAL and 1µg HA-MARCH2. Twenty-four hours after transfection, cells were fixed and subjected to indirect fluorescent immunocytochemical staining with an anti-HA monoclonal antibody followed by Cy3-conjugated secondary antibody. HA-MARCH2 appears in red and GFP-STX6 and GFP-CAL are green. Arrow heads point to colocalization. (<b>B</b>) HEK293 cells were co-transfected with 3µg myc-CAL, 3µg myc-STX6, and 1µg HA-MARCH2 as indicated. After 48 h, cell lysates were harvested and immunoprecipitated with an anti-HA affinity matrix. Cell lysates and immunoprecipitated materials were subjected to immunoblot analysis with Myc or HA antibodies. Data shown are representative of at least three independent experiments.