Co-localization of MARCH2, STX6, and CAL.

<p>HEK293 cells grown on coverslips were co-transfected with 3µg GFP-STX6, 3µg myc-CAL, and 1µg HA-MARCH2. After 24 h, cells were fixed and subjected to indirect fluorescent immunocytochemical staining with an anti-myc rabbit polylonal antibody and an anti-HA mouse monoclonal antibody followed by goat anti-rabbit Alexa Fluor 647-conjugated secondary antibody and goat anti-mouse Alexa Fluor 594-conjugated secondary antibody. (<b>A</b>) GFP-STX6 appears in green. (<b>B</b>) HA-MARCH2 appears in red. (<b>C</b>) myc-CAL appears in magenta. (<b>D</b>) Overlay of GFP-STX6, HA-MARCH2 and myc-CAL. Data shown are representative of at least three independent experiments.