UiFC fluorescence colocalize well with K48 chains but not K63 chains enriched in p62/SQSTM1 bodies.
- Zhiliang Chen (454388)
- Yongwang Zhong (207471)
- Yang Wang (5921)
- Shan Xu (454389)
- Zheng Liu (28397)
- Ilia V. Baskakov (150840)
- Mervyn J. Monteiro (207483)
- Mariusz Karbowski (454390)
- Yuxian Shen (454391)
- Shengyun Fang (207485)
Publication date
September 2013
DOIAbstract
<p>K63 chains colocalize well with p62/SQSTM1 bodies (lower left) but not with UiFC fluorescence (lower right). HeLa cells transfected with plasmids encoding HA-p62/SQSTM1 and UiFC were processed for immunostaining with mouse monoclonal anti-p62/SQSTM1 (blue) and rabbit monoclonal anti-K63 chain antibody (red) (A) or affinity-purified rabbit polyclonal anti-p62/SQSTM1 (blue, pseudo-colored to cyan) and mouse monoclonal anti-K48 chain antibody (red) (B), followed by fluorescent microscopy.</p
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