Detection for dimerization of yeast truncated Ste2p lacking the carboxy-terminal tail (Ste2ΔC) receptor in NMY62 strain.

<p>Growth and quantitative β-galactosidase activity of yeast cells expressing various combinations of Cub and Nub<i>G</i> fusions. The control bait plasmid was pBT3-C mock vector (empty vector). The control prey plasmids were pPR3-C mock vector (empty vector) and pPR3-HXT1. (A) Growth assay without α-factor (<i>left panels</i>) and with 5 µM α-factor (<i>right panels</i>). Each cell was spotted in serial 10-fold dilutions on SD –Leu, Trp, Ade and His dropout plate. (B) Quantitative β-galactosidase assay. Error bars represent the standard deviations (<i>n = </i>3).