CFH SCR7 402H variant binds Fib3 with higher affinity than the Y variant in Y2H.

<p>Yeast clones containing the Fib3 prey construct and either the CFH 402H (CFH7H) or 402Y (CFH7Y) bait constructs were tested in quantitative β-galactosidase assay. Controls shown are CFH7H bait with empty prey vector (pGAD) and Fib3 prey with empty bait vector (pGBTK7). Activity is expressed in Miller Units: ( = 1000×OD₅₇₈/t×V×OD₆₀₀, where t = incubation time in min; V = 0.1 concentration factor). Standard deviation error bars (N = 8) are shown.