Ryp1, Ryp2, and Ryp3 physically interact.

<p>(A) Co-IP experiments were performed in wild-type cells grown at 37°C using Ryp2 and Ryp3 antibodies. Immunoblot shows detection of Ryp1, Ryp2, and Ryp3 in the input and elution fractions of Ryp2 and Ryp3 IPs. (B, C) Yeast-two-hybrid matings were performed with bait and prey strains listed. Diploid cells were spotted onto minimal media plates: -UHT, -Uracil/-Histidine/-Tryptophan; –UHTL, -Uracil/-Histidine/-Tryptophan/-Leucine. Growth on -UHTL plates and blue color on -UHT+X-gal plates indicate an interaction between proteins expressed in bait and prey strains. β-galactosidase activities were measured for three independent isolates of each strain. Quadruplicate measurements of representative isolates are graphed as mean ± standard deviation.