Detection for dimerization of yeast Ste2p deletion mutants (TM1–5 and TM6–7) in NMY62 strain.

<p>(A) Schematic of Ste2ΔC and the deletion mutants. Transmembrane (TM) domains are indicated with pillar-type boxes, and the Cub (Cub-LexA-VP16) or Nub<i>G</i> (Nub with I13G mutation) is depicted as a rounded rectangle. (B) Growth assay without α-factor (<i>left panels</i>) and with 5 µM α-factor (<i>right panels</i>). Each cell was spotted in serial 10-fold dilutions on SD –Leu, Trp, Ade and His dropout plate. (C) Quantitative β-galactosidase activity in yeast cells containing various combinations of plasmids. Error bars represent the standard deviations (<i>n = </i>3). The control prey plasmids were pPR3-C mock vector (empty vector) and pPR3-HXT1.