Electroretinography analysis for WT and <i>CFB</i>^−/− mice following CE.

<p>ERG recordings were performed in cohorts of age-matched WT (<b>A</b>) and <i>CFB ^−/−</i> (<b>B</b>) mice exposed to 6 months of cigarette smoke (CE) or room air. Dark-adapted, scotopic ERGs were recorded in response to increasing light intensities, and light-adapted photopic UV-cone ERGs to a single, maximum light intensity. Scotopic ERGs were analyzed using multiple ANOVA, followed by comparison of individual light intensities using <i>t</i>-test analyses. WT mice exposed to smoke had significantly lower dark-adapted b-wave amplitudes compared to controls (<i>P</i><0.001), in particular at higher light intensities (20, 10, 6, 0 dB). Impairment in cone function is evidenced by the reduction in maximum UV-cone b-wave amplitudes. In comparison, ERG amplitudes in both scotopic and photopic ERGs were unaffected in AP-deficient mice exposed to smoke. Photoreceptor cell responses (a-waves), which drive the b-waves, were equally affected (data not shown). Data are expressed as mean ±SEM (n = 6–8 per condition; *, <i>P</i><0.05; **, <i>P</i><0.001).