Suppression of motility defects in Δ<i>ssaI</i> and Δ<i>ssaR</i> mutants by CtrA.

<p><i>P_lac-ctrA</i> plasmid (pJZ008) was conjugated into Δ<i>ssaI</i> and Δ<i>ssaR</i> mutants, respectively. The conjugants were selected and inoculated on swim agar plates for 8 days at 28°C. 200 µM IPTG was added to the media. The Δ<i>ssaI</i> mutant complemented with <i>P_lac-ssaI</i> (pEC108) and the Δ<i>ssaR</i> mutant with <i>P_lac-ssaR</i> (pEC112) were used as positive controls. Wild type KLH11 (EC1) was used as a positive and the Δ<i>ssaI</i> and Δ<i>ssaR</i> strains were used as negative control. The results were representatives of several independent experiments each with three biological replicates.