<p>CD4<sup>+</sup>Foxp3<sup>+</sup> cells isolated from naïve mice and transfected with miR-182 mimics or hairpin inhibitors (A) or miR-10a mimics or hairpin inhibitors (B) to identify predicted target gene regulation. RNA was extracted 24 hours post transfection for analysis. One of 3 individual experiments shown, with 3 biological replicates in each experiment. p-value = 0.05. with data expressed as mean ±SEM.</p
Summary RNA interference is a powerful tool for studying gene function, however, the reproducible ge...
Abstract Gene regulation is vital for life and it involves plethora of mechanisms including microRNA...
<p>(<b>A</b>) Fluorescent and merged images of A549, SK-LU1 and NP-69 cells transfected with miRIDIA...
<p>(A) SHSY5Y cells were transfected with hairpin inhibitors for human miR-200c, miR-182, miR-183 an...
<p>Total RNA was isolated from h1299 cells at 48 h after transfection with miRNA mimics or miRNA hai...
<p>RNA samples for genome-wide gene expression analysis were collected 24 hours after MDA-MB-231(SA)...
<p>(A) mRNA levels of ELK3 target genes, measured by RT-QPCR, 24 h after treatment with either siELK...
<p>(A) MMP-2 and (B) PIK3R1 were significantly suppressed by transfection of the respective miRNA mi...
<p>A. The region of the rat TRPV4 mRNA 3′UTR predicted to be targeted by miR-203. Three different bi...
<p>(A–D) Real-Time RT-PCR of Timp3, Rbm24, Tgfbr2 and Csnk2a2 in HPASM cells transfected with mimics...
<p>(<b>a</b>) Rationale of the assay. (<b>b</b>) Unbiased identification of phenotypically important...
SummaryRNA interference is a powerful tool for studying gene function, however, the reproducible gen...
<p>To confirm the functional significance of epididymal miRNAs, an immortalized mouse caput epididym...
<p>(A) The alignment of miR223 and its target sites in 3′-UTR of STAT3 is shown. (B) RAW264.7 cells ...
I have developed a system in mouse embryonic stem (ES) cells to simply and rapidly derive gene lists...
Summary RNA interference is a powerful tool for studying gene function, however, the reproducible ge...
Abstract Gene regulation is vital for life and it involves plethora of mechanisms including microRNA...
<p>(<b>A</b>) Fluorescent and merged images of A549, SK-LU1 and NP-69 cells transfected with miRIDIA...
<p>(A) SHSY5Y cells were transfected with hairpin inhibitors for human miR-200c, miR-182, miR-183 an...
<p>Total RNA was isolated from h1299 cells at 48 h after transfection with miRNA mimics or miRNA hai...
<p>RNA samples for genome-wide gene expression analysis were collected 24 hours after MDA-MB-231(SA)...
<p>(A) mRNA levels of ELK3 target genes, measured by RT-QPCR, 24 h after treatment with either siELK...
<p>(A) MMP-2 and (B) PIK3R1 were significantly suppressed by transfection of the respective miRNA mi...
<p>A. The region of the rat TRPV4 mRNA 3′UTR predicted to be targeted by miR-203. Three different bi...
<p>(A–D) Real-Time RT-PCR of Timp3, Rbm24, Tgfbr2 and Csnk2a2 in HPASM cells transfected with mimics...
<p>(<b>a</b>) Rationale of the assay. (<b>b</b>) Unbiased identification of phenotypically important...
SummaryRNA interference is a powerful tool for studying gene function, however, the reproducible gen...
<p>To confirm the functional significance of epididymal miRNAs, an immortalized mouse caput epididym...
<p>(A) The alignment of miR223 and its target sites in 3′-UTR of STAT3 is shown. (B) RAW264.7 cells ...
I have developed a system in mouse embryonic stem (ES) cells to simply and rapidly derive gene lists...
Summary RNA interference is a powerful tool for studying gene function, however, the reproducible ge...
Abstract Gene regulation is vital for life and it involves plethora of mechanisms including microRNA...
<p>(<b>A</b>) Fluorescent and merged images of A549, SK-LU1 and NP-69 cells transfected with miRIDIA...