TRPV4 is a target of miR-203 in HSC.

<p>A. The region of the rat TRPV4 mRNA 3′UTR predicted to be targeted by miR-203. Three different bioinformatics approaches (miRbase, miRANDA, and Targetscan 5.1) were used for the target prediction. B. The TRPV4 mRNA expression was analyzed by real-time PCR. The results are expressed as relative expression against control expression. *P<0.05 vs control(non-treated cells), #P<0.05 vs model(TGF-β1-treated cells).C. Dual-luciferase reporter assays of TRPV4 in HSC-T6 cells. Reporter construct containing wt TRPV4 3′-UTR was cotransfected with miR-203 mimics or NC-miRNA. Relative luciferase activity was normalized to firefly luciferase. Data are representative of at least three separate experiments. *P<0.05, **P<0.01vs. NC-miRNA.