rESAT-6-CFP10 induced IL-4 responses in tuberculosis patients and healthy<i>M.bovis</i> BCG vaccinated healthy donors.

<p>PBMC obtained from 10 active TB patients and 17 healthy donors were cultured in the presence of rESAT-6-CFP10 or PHA for 6 days. Cells were then stained to detect the presence of intracellular IL-4. The figure represents the overall summary for rESAT-6-CFP10 (A & B) and PHA responses (C & D) in CD4⁺ (A & C) and CD8⁺ (B & D) T cells. The bars represent the means and the error bars are 95% CI. P-values were obtained from the Mann-Whitney test for comparison of healthy donors with T0 and comparisons were made between T0 and other time points for TB patients using the non-parametric repeated measures one-way ANOVA (Friedman’s test) with Dunn’s multiple comparison (**P<0.05 in longitudinal comparison and * P<0.05 in unpaired variables ). Abbreviations are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068121#pone-0068121-g001" target="_blank"><b>Figure 1</b></a>.