An illustration of the trend in rESAT-6-CFP10-induced regulatory T cell expression and IFN-γ/IL-4 ratios in the intensive phase of TB treatment.

<p>Freshly isolated PBMC from 10 TB patients were cultured in the presence of ESAT-6-CFP10 or without antigen for 6 days. Harvested cells were then stained for the expression of CD25, Foxp3, IFN-γ and IL-4. The mean regulatory T cell expression is plotted against the mean of the ratio of IFN-γ/IL-4 expressing T cells at diagnosis and during follow up of TB patients. Abbreviations are explained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068121#pone-0068121-g001" target="_blank"><b>Figure 1</b></a>.