Expression of SAMHD1 in vaccinia-infected MDMs.
- Joseph A. Hollenbaugh (117029)
- Peter Gee (427956)
- Jonathon Baker (427957)
- Michele B. Daly (427958)
- Sarah M. Amie (427959)
- Jessica Tate (427960)
- Natsumi Kasai (427961)
- Yuka Kanemura (427962)
- Dong-Hyun Kim (117030)
- Brian M. Ward (427963)
- Yoshio Koyanagi (288992)
- Baek Kim (66773)
Publication date
June 2013
DOIAbstract
<p>(A) MDMs treated with Vpx+ or Vpx− VLP were infected (1 PFU/cell) with either WR or WRΔTK virus (NI: no infection). Cell lysates were generated 24 hpi and analyzed by Western blot using α-SAMHD1, α-actin, or α-GFP antibodies. (A) Representative immunoblots from one donor are shown. Immunoblots for SAMHD1 (B) and GFP (C) were quantified for three independent donors. Data were normalized to the amount of actin signal and were plotted for the amount of signal in the NI lane (set to 1.0) for SAMHD1 and the Vpx− VLP lane (set to 1.0) for GFP.</p
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