Effects of SAMHD1 region deletion variants on Vpx-induced degradation.

<p>(A) Immunoblot of SAMHD1 WT and deletion mutants in the presence and absence of Vpx. HA-tagged SAMHD1 constructs were co-expressed with HA-tagged Vpx or empty vector in 293T cells. Cell extracts were harvested 48 h later and analyzed by SDS-PAGE, followed by immunoblotting to detect SAMHD1-HA and HA-Vpx; β-actin was used as the loading control. (B) The bar graph shows the percentages of relative band intensity for SAMHD1 in the presence of Vpx, vs. in the absence of Vpx.