Vaccinia replication in MDMs.

<p>(A) Human primary monocyte-derived macrophages (MDMs) were pretreated with VLPs: Vpx+ and Vpx− for 24 h prior to infection with Western Reserve (WR) stain of vaccinia virus expressing the viral core protein A4 fused to YFP. A representative FACS dot blot shows the percent of infected MDMs at 24 hpi. (B) The means of three independent donors were graphed and are displayed as the percentages of YFP+ MDMs for the different VLPs treatment groups. (C) Plaque forming units (PFUs) were determined for three donors as described in Experimental Procedures. The fold changes between Vpx+ VLP and Vpx− VLP groups are displayed.