GFP-dnMAML fusion protein is expressed in dnMAML mice and inhibits Notch signaling during fracture healing.

<p>(<b>A</b>) GFP gene expression is upregulated in dnMAML fractures. (<b>B</b>) This correlates with a 30% reduction in Hes1 gene expression at 5dpf. (<b>C</b>) GFP is expressed in undifferentiated mesenchymal cells, chondrocytes, osteoblasts, endothelial cells, hematopoietic cells and inflammatory cells in dnMAML fractures. There is no expression in WT mice. GFP IHC images were acquired at 200x magnification. Gene expression data is presented as fold change to WT for each time point, calculated using the formula. 2^-ΔΔC(t). *p<0.050 (dnMAML vs WT).