dnMAML decreases osteogenic and osteoclast cell density.

<p>(<b>A</b>) dnMAML expression decreases osteoblast density normalized by bone perimeter (Obl/BP) and (<b>B</b>) increases osteocyte density normalized by bone area (Ocy/BA) at 20dpf, (<b>C</b>) which results in an increased osteocyte-to-osteoblast ratio (Ocy:Obl). (<b>D</b>) However, dnMAML expression decreases overall osteogenic (osteoblast and osteocyte) cell density normalized by bone area (Osteo/BA) at 20dpf. (<b>E</b>) Osteocalcin (Ocn) gene expression is increased in dnMAML fractures at 20dpf. There are no differences in (<b>F</b>) osterix (Osx) or (<b>G</b>) collagen type I (Col1a1) gene expression. (<b>H</b>) dnMAML expression decreases osteoclast density normalized by bone area (Ocl/BA) and (<b>I</b>) TRAP gene expression at 20dpf. Masson’s Trichrome images were acquired at 200x and 400x magnification for cell-based histomorphometric analysis. Gene expression data is presented as relative expression to β-actin, calculated using the formula. 2^-ΔC(t). *p<0.050 (dnMAML vs WT).