The final concentration of IS6110 DNA in the eluate following magnetic bead-based extraction of 3 and 5 mL spiked urine samples is significantly higher than the IS6110 DNA concentration in the eluate following the extraction of 1 mL samples (mean ± s.d., n = 3); * denotes statistically different from 1 mL sample.

Comparison of five automated DNA extraction systems.

Carina Heydt (611868)
Jana Fassunke (611869)
Helen Künstlinger (611870)
Michaela Angelika Ihle (611871)
Katharina König (315427)
Lukas Carl Heukamp (611872)
Hans-Ulrich Schildhaus (415250)
Margarete Odenthal (180129)
Reinhard Büttner (86398)
Sabine Merkelbach-Bruse (611873)

August 2014

Illustration of relative DNA concentrations of samples 1–10 measured by the NanoDrop 2000c spectr...

There is no significant difference in DNA recovery using the lyophilized urine collection pipette stored for 0, 4, 8, or 12 weeks (black circles) compared to freshly prepared adsorption reagents (white circle) (mean ± s.d., n = 3,).

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

There is no significant difference in DNA recovery using the lyophilized urine collection pipette...

Log-transformed DNA concentration (ng μl-1) in saliva extracts.

Diana Lobo (817584)
Raquel Godinho (372545)
Francisco Álvares (817585)
José V. López-Bao (232369)
Alejandro Rodríguez (4243183)

October 2015

Means and 95% CI are shown for every combination of substrate and bait, once records >60 ng μl<su...

The percentage of DNA recovered from 1 mL urine samples increases as the concentration of magnetic beads increases to 6×108 beads/mL (mean ± s.d., n = 3).

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

The percentage of DNA recovered from 1 mL urine samples increases as the concentration of magneti...

The DNA recovery for the magnetic bead-based extraction method and a commercially available laboratory-based kit are comparable.

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

Though both are significantly higher than the unextracted sample, which is undetectable by PCR, t...

Numbers of Pt-DACH molecules per one DNA molecule (NPt).

Hong-Yan Zhang (444630)
Yu-Ru Liu (134590)
Chao Ji (276390)
Wei Li (7081)
Shuo-Xing Dou (134593)
Ping Xie (112003)
Wei-Chi Wang (134592)
Ling-Yun Zhang (139795)
Peng-Ye Wang (134595)

August 2013

The concentration of Pt-DACH was 60 µM. Each data point was the mean of four independent measurem...

A magnetic bead-based method for concentrating DNA from human urine for downstream detection.

Hali Bordelon
Patricia K Russ
David W Wright
Frederick R Haselton

Due to the presence of PCR inhibitors, PCR cannot be used directly on most clinical samples, includi...

DNA amount measured at 260 nm after DNA extractions (a: van Soolingen procedure, b: Invisorb Spin Tissue Mini Kit from Invitek, c: PowerSoil DNA isolation Kit from MoBio) from big (+++: 10 log10 cells), medium (++: 9 log10 cells), and small (+: 8 log10 cells) pellets of MAP K-10 (A, n=54), and M. tuberculosis H37Ra (B, n=54) cells.

Nicolas Radomski (475617)
Louis Kreitmann (475618)
Fiona McIntosh (285455)
Marcel A. Behr (88762)

October 2013

Error bars are standard deviation. LOQ are limit of quantification of the spectrophotometer.</p

Total DNA amount, OD* values at 260 nm and standard quality scores per sample and extraction method.

Sabine Telle (376090)
Marco Thines (298658)

February 2013

*OD260 nm of 1 equals 50 ng/µl.adilution 1∶10.bhighly overestimated DNA ...

Significant discrepancies in DNA quantification by NanoDrop and Qubit.

Michele Simbolo (418972)
Marisa Gottardi (418973)
Vincenzo Corbo (242342)
Matteo Fassan (221852)
Andrea Mafficini (418974)
Giorgio Malpeli (324076)
Rita T. Lawlor (418975)
Aldo Scarpa (242345)

June 2013

A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements w...

DNA yield from eight DNA extraction kit-filter paper combinations from samples in aquaria with UV-sterilized water.

Rheyda Hinlo (4102933)
Dianne Gleeson (538082)
Mark Lintermans (3367358)
Elise Furlan (528651)

June 2017

Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. CN = Cellul...

Scatterplot of time vs. extracted DNA amount (μg).

Hasan Alhaddad (106573)
Tasneem Maraqa (6282035)
Suha Alabdulghafour (6282038)
Huda Alaskar (6282041)
Randa Alaqeely (6282044)
Faisal Almathen (4342366)
Bader H. Alhajeri (6282047)

January 2019

Samples are separated by DNA source (saliva vs. tail-hair). The amount of DNA is that obtained from ...

Comparison of DNA yields of type strains obtained using six DNA extraction methods.

Sanqing Yuan (175642)
Dora B. Cohen (175645)
Jacques Ravel (5502)
Zaid Abdo (175649)
Larry J. Forney (175653)

March 2012

aDNA concentrations are means calculated using data from eight replicates.bMeans with the ...

Linearity.

Frederik Banch Clausen (11657806)
Kristine Mathilde Clara Lund Jørgensen (14675941)
Lasse Witt Wardil (14675944)
Leif Kofoed Nielsen (14675947)
Grethe Risum Krog (14675950)

February 2023

(A) Showing measured absolute values for DNA concentrations ranging from 0.15 to 400 copies/μL (n = ...

Sensitivity of the LAMP assay in simulated human urine samples artificially contaminated with DNA from S. haematobium.

Javier Gandasegui (778298)
Pedro Fernández-Soto (403075)
Cristina Carranza-Rodríguez (778299)
José Luis Pérez-Arellano (403078)
Belén Vicente (615664)
Julio López-Abán (134057)
Antonio Muro (134062)

July 2015

(A) Sensitivity assessment of LAMP when performing the DNA extraction with the i-genomic Urine DN...

Comparison of five automated DNA extraction systems.

Carina Heydt (611868)
Jana Fassunke (611869)
Helen Künstlinger (611870)
Michaela Angelika Ihle (611871)
Katharina König (315427)
Lukas Carl Heukamp (611872)
Hans-Ulrich Schildhaus (415250)
Margarete Odenthal (180129)
Reinhard Büttner (86398)
Sabine Merkelbach-Bruse (611873)

August 2014

Illustration of relative DNA concentrations of samples 1–10 measured by the NanoDrop 2000c spectr...

There is no significant difference in DNA recovery using the lyophilized urine collection pipette stored for 0, 4, 8, or 12 weeks (black circles) compared to freshly prepared adsorption reagents (white circle) (mean ± s.d., n = 3,).

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

There is no significant difference in DNA recovery using the lyophilized urine collection pipette...

Log-transformed DNA concentration (ng μl-1) in saliva extracts.

Diana Lobo (817584)
Raquel Godinho (372545)
Francisco Álvares (817585)
José V. López-Bao (232369)
Alejandro Rodríguez (4243183)

October 2015

Means and 95% CI are shown for every combination of substrate and bait, once records >60 ng μl<su...

The percentage of DNA recovered from 1 mL urine samples increases as the concentration of magnetic beads increases to 6×108 beads/mL (mean ± s.d., n = 3).

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

The percentage of DNA recovered from 1 mL urine samples increases as the concentration of magneti...

The DNA recovery for the magnetic bead-based extraction method and a commercially available laboratory-based kit are comparable.

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

Though both are significantly higher than the unextracted sample, which is undetectable by PCR, t...

Numbers of Pt-DACH molecules per one DNA molecule (NPt).

Hong-Yan Zhang (444630)
Yu-Ru Liu (134590)
Chao Ji (276390)
Wei Li (7081)
Shuo-Xing Dou (134593)
Ping Xie (112003)
Wei-Chi Wang (134592)
Ling-Yun Zhang (139795)
Peng-Ye Wang (134595)

August 2013

The concentration of Pt-DACH was 60 µM. Each data point was the mean of four independent measurem...

A magnetic bead-based method for concentrating DNA from human urine for downstream detection.

Hali Bordelon
Patricia K Russ
David W Wright
Frederick R Haselton

Due to the presence of PCR inhibitors, PCR cannot be used directly on most clinical samples, includi...

DNA amount measured at 260 nm after DNA extractions (a: van Soolingen procedure, b: Invisorb Spin Tissue Mini Kit from Invitek, c: PowerSoil DNA isolation Kit from MoBio) from big (+++: 10 log10 cells), medium (++: 9 log10 cells), and small (+: 8 log10 cells) pellets of MAP K-10 (A, n=54), and M. tuberculosis H37Ra (B, n=54) cells.

Nicolas Radomski (475617)
Louis Kreitmann (475618)
Fiona McIntosh (285455)
Marcel A. Behr (88762)

October 2013

Error bars are standard deviation. LOQ are limit of quantification of the spectrophotometer.</p

Total DNA amount, OD* values at 260 nm and standard quality scores per sample and extraction method.

Sabine Telle (376090)
Marco Thines (298658)

February 2013

*OD260 nm of 1 equals 50 ng/µl.adilution 1∶10.bhighly overestimated DNA ...

Significant discrepancies in DNA quantification by NanoDrop and Qubit.

Michele Simbolo (418972)
Marisa Gottardi (418973)
Vincenzo Corbo (242342)
Matteo Fassan (221852)
Andrea Mafficini (418974)
Giorgio Malpeli (324076)
Rita T. Lawlor (418975)
Aldo Scarpa (242345)

June 2013

A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements w...

DNA yield from eight DNA extraction kit-filter paper combinations from samples in aquaria with UV-sterilized water.

Rheyda Hinlo (4102933)
Dianne Gleeson (538082)
Mark Lintermans (3367358)
Elise Furlan (528651)

June 2017

Clear bars used DNeasy extraction kit while shaded grey bars used the PowerWater kit. CN = Cellul...

Scatterplot of time vs. extracted DNA amount (μg).

Hasan Alhaddad (106573)
Tasneem Maraqa (6282035)
Suha Alabdulghafour (6282038)
Huda Alaskar (6282041)
Randa Alaqeely (6282044)
Faisal Almathen (4342366)
Bader H. Alhajeri (6282047)

January 2019

Samples are separated by DNA source (saliva vs. tail-hair). The amount of DNA is that obtained from ...

Comparison of DNA yields of type strains obtained using six DNA extraction methods.

Sanqing Yuan (175642)
Dora B. Cohen (175645)
Jacques Ravel (5502)
Zaid Abdo (175649)
Larry J. Forney (175653)

March 2012

aDNA concentrations are means calculated using data from eight replicates.bMeans with the ...

Linearity.

Frederik Banch Clausen (11657806)
Kristine Mathilde Clara Lund Jørgensen (14675941)
Lasse Witt Wardil (14675944)
Leif Kofoed Nielsen (14675947)
Grethe Risum Krog (14675950)

February 2023

(A) Showing measured absolute values for DNA concentrations ranging from 0.15 to 400 copies/μL (n = ...

Sensitivity of the LAMP assay in simulated human urine samples artificially contaminated with DNA from S. haematobium.

Javier Gandasegui (778298)
Pedro Fernández-Soto (403075)
Cristina Carranza-Rodríguez (778299)
José Luis Pérez-Arellano (403078)
Belén Vicente (615664)
Julio López-Abán (134057)
Antonio Muro (134062)

July 2015

(A) Sensitivity assessment of LAMP when performing the DNA extraction with the i-genomic Urine DN...

Comparison of five automated DNA extraction systems.

Carina Heydt (611868)
Jana Fassunke (611869)
Helen Künstlinger (611870)
Michaela Angelika Ihle (611871)
Katharina König (315427)
Lukas Carl Heukamp (611872)
Hans-Ulrich Schildhaus (415250)
Margarete Odenthal (180129)
Reinhard Büttner (86398)
Sabine Merkelbach-Bruse (611873)

August 2014

Illustration of relative DNA concentrations of samples 1–10 measured by the NanoDrop 2000c spectr...

There is no significant difference in DNA recovery using the lyophilized urine collection pipette stored for 0, 4, 8, or 12 weeks (black circles) compared to freshly prepared adsorption reagents (white circle) (mean ± s.d., n = 3,).

Hali Bordelon (431546)
Patricia K. Russ (232554)
David W. Wright (361828)
Frederick R. Haselton (232572)

July 2013

There is no significant difference in DNA recovery using the lyophilized urine collection pipette...

Log-transformed DNA concentration (ng μl-1) in saliva extracts.

Diana Lobo (817584)
Raquel Godinho (372545)
Francisco Álvares (817585)
José V. López-Bao (232369)
Alejandro Rodríguez (4243183)

October 2015

Means and 95% CI are shown for every combination of substrate and bait, once records >60 ng μl<su...

Abstract

Extracted data

Abstract

Extracted data

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