Effect of leptin on the expression of SP-A in fetal lungs and type-II AECs.

<p>A: Fetal lung tissue from the sham control group, PBS-treated FGR group, and leptin-treated FGR group were collected and total RNA was extracted for mRNA expression measurement of surfactant protein A (black), B (dark gray), C (light gray) and D (white) by quantitative real-time RT-PCR. <i>^*P</i><0.01 <i>vs</i> the PBS-treated FGR group (<i>n</i> = 3). B: Fetal lung tissue isolated from the sham control group (lane 1), PBS-treated FGR group (lane 2), and leptin-treated FGR group (lane 3) were subjected to western blot analysis to determine the protein levels of surfactant protein A, B, C and D, which were semi-quantified by densitometry and normalized by β-actin. Data are presented as mean ± SEM. <i>^*P</i><0.01, <i>^#P</i><0.05 <i>vs</i> the PBS-treated FGR group (<i>n</i> = 3). C: Cultured type-II AECs were exposed to normal conditions or hypoxia for 12 h with different concentrations of leptin or specific antibody against leptin. Whole lysates were detected by immunoblotting with anti-SP-A antibody. <i>^*P</i><0.01, <i>^#P</i><0.05; a: <i>vs</i> the hypoxia-treated group, b: <i>vs</i> the 1600 ng/mL leptin-treated group (<i>n</i> = 3).