Expression of leptin and Lep-R in fetal lungs of the FGR rat.

<p>A and B: Leptin (A) and Lep-R (B) in fetal lungs at embryonic day 17 (E17), day 19 (E19) and day 21 (E21) were determined by immunohistochemical staining (×400). NC: the negative control. C: Fetal lung tissue from different stages of FGR rats was collected, and total RNA was extracted for mRNA expression measurement of leptin (right) and Lep-R (left) by quantitative real-time RT-PCR. <i>^*P</i><0.01, <i>^#P</i><0.05; a: <i>vs</i> E17 sham group, b: <i>vs</i> the corresponding sham group (<i>n</i> = 3). D: Fetal lung tissue isolated from different stages of FGR and sham control rats were subjected to western blot analysis to determine the protein levels of leptin and leptin receptor. All data were shown as means of three independent experiments.