Antioxidant effects of DHA in HUVECs.

<p>(A) HUVECs were pretreated with DHA for 16 h, and then stimulated with tBHP (250 or 500 µM) for 6 h. Cell viability was determined by MTT assay. Values are expressed as percentage of cell survival, and each represents the mean ± SE of 4 experiments. (B, C) HUVECs were incubated with DHA (75 µM) for 16 h. GSH concentration (B) and ratio of GSH/GSSG (C) were determined. Each value represents the mean ± SE of 7 experiments. (D) HUVECs were pretreated with DHA (75 µM) for 16 h, and exposed to tBHP (250 or 500 µM). ROS released from cells was determined at different time intervals over 2-h period. Each value represents the mean ± SE of 8 experiments. ***<i>P</i><0.001, compared with tBHP-treated BSA control, <b>^†††</b><i>P</i><0.001, compared with tBHP-untreated BSA control.