DHA induces ROS accumulation in MCF-7 cells.

<p>(<b>A</b>) Cell viability (MTT assay) of MCF-7 cells treated with different concentrations of α-tocoperol, ascorbic acid, or N-acetylcysteine (NAC) and DHA. Each value is the mean ± S.E. (n = 6). (<b>B</b>) ROS accumulation measured by H₂-DCF-DA staining of MCF-7 cells treated with 25 µM DHA or together with 1 µM α-tocoperol for indicated time periods. The pictures were taken under a fluorescent microscope at the 100× magnification. (<b>C</b>) 3-Nitrotyrosine staining of MCF-7 cells treated with 25 µM DHA or together with 1 µM α-tocoperol for indicated time periods. The pictures were taken under a fluorescent microscope at the 400× magnification. Each value is the mean ± S.E. (n = 3).